LIC Generator, a resource to generate primers for Ligation Independent Cloning | [ Mirror site ]

Restriction no more !

Links
Primer design for:
  • LIC-PCR
  • Mixed-PCR
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  • Detection of internal LIC sequences
  • Primer design at Clontech (In-fusion)
  • All about LIC
  • LIC protocols
  • sLIC at Bitesize Bio

Introduce tags or protease cleavage sites into your insert and vector

1- Choose the features of your LIC1 sequence (5' to your insert)

Tag/Protease

2- Choose the features of your LIC2 sequence (3' to your insert)

Tag/Protease
Credits: The informatics team at the IBS Grenoble, 41, rue Jules Horowitz 38027 Grenoble Cedex 1 France  
Author: Marie-Claire Dagher
New address : Centre Diagnostic et Recherche CGD, 6ème étage, Aile E , CHU Grenoble, BP 217, 38043 Grenoble Cedex 9, France, Office Phone: 00-33-4-76765483, Email : mcdagher(at)chu-grenoble(dot)fr, logo cea    logo cnrs    logo Universite Joseph Fourier